Amino acids differ in their capacity to stimulate GLP-1 release from the perfused rat small intestine and stimulate secretion by different sensing mechanisms
Ida Marie Modvig, Rune E. Kuhre, Sara L. Jepsen, Stella Feng Sheng Xu, Maja S. Engelstoft, Kristoffer L. Egerod, Thue W. Schwartz, Cathrine Ørskov, Mette M. Rosenkilde, Jens J. Holst
Abstract
Using isolated perfused rat small intestines, we show that amino acids differ in their mechanisms and capacity of stimulating GLP-1 release. Furthermore, we demonstrate that sensing by GPR142, GPR35, GPR93, and the umami taste receptor (Tas1R1/Tas1R3) are not involved in amino acid stimulated GLP-1 release. In contrast to previous studies, this experimental model allows discrimination between the luminal and the vascular side of the intestine, which is essential when studying mechanisms of amino acid-stimulated GLP-1 secretion.
Topics & Concepts
SecretionSmall intestineChemistryAmino acidCell biologyBiochemistryEndocrinologyInternal medicineBiologyMedicineBiochemical Analysis and Sensing TechniquesDiet and metabolism studiesDiabetes Treatment and Management