Silk Hydrogel Substrate Stress Relaxation Primes Mesenchymal Stem Cell Behavior in 2D
Suttinee Phuagkhaopong, Luís Mendes, K. H. Müller, Manja Wobus, Martin Bornhäuser, H Carswell, Iola F. Duarte, F. Philipp Seib
Abstract
signaling in response to hydrogel mechanics. An elastic substrate also induced higher consumption of glucose and aspartate, coupled with a higher secretion of lactate, than was observed in MSCs grown on viscoelastic substrate. However, both silk hydrogels changed the magnitude of consumption of glucose, pyruvate, glutamine, and aspartate, and also metabolite secretion, resulting in an overall lower metabolic activity than that found in control cells. Together, these findings describe how stress relaxation impacts the overall biology of MSCs cultured on silk hydrogels.
Topics & Concepts
Materials scienceMesenchymal stem cellStress relaxationSubstrate (aquarium)Self-healing hydrogelsSILKNanotechnologyRelaxation (psychology)Stress (linguistics)BiophysicsComposite materialChemical engineeringPolymer chemistryCell biologyNeuroscienceCreepBiologyEcologyLinguisticsEngineeringPhilosophySilk-based biomaterials and applicationsElectrospun Nanofibers in Biomedical ApplicationsRNA Interference and Gene Delivery