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HIV-1 Integrase Inhibitors That Are Active against Drug-Resistant Integrase Mutants

Steven J. Smith, Xue Zhi Zhao, Dario Oliveira Passos, Dmitry Lyumkis, Terrence R. Burke, Stephen H. Hughes

2020Antimicrobial Agents and Chemotherapy32 citationsDOIOpen Access PDF

Abstract

Thus, it is important to develop new and improved INSTIs that are effective against all the known resistant mutants. This led us to test our best inhibitors, in parallel with DTG, BIC, and CAB, in a single-round infection assay against a panel of the new CAB-resistant mutants. Of the INSTIs we tested, BIC and our compound 4d had the broadest efficacy. Both were superior to DTG, as evidenced by the data obtained with the IN mutant T66I/L74M/E138K/S147G/Q148R/S230N, which was selected by CAB using an EVG-resistant lab strain. These results support the preclinical development of compound 4d and provide information that can be used in the design of additional INSTIs that will be effective against a broad spectrum of resistant mutants.

Topics & Concepts

DolutegravirElvitegravirRaltegravirIntegraseVirologyIntegrase inhibitorNucleoside Reverse Transcriptase InhibitorReverse transcriptaseBiologyPharmacologyHuman immunodeficiency virus (HIV)Antiretroviral therapyViral loadGeneticsRNAGeneHIV/AIDS drug development and treatmentBiochemical and Molecular ResearchHIV Research and Treatment