Biosynthetic gene cluster identification and biological activity of lucilactaene from <i>Fusarium</i> sp. RK97-94
Sho Kato, Takayuki Motoyama, Yushi Futamura, Masakazu Uramoto, Toshihiko Nogawa, T. Hayashi, Hiroshi Hirota, Akira Tanaka, Naoko Takahashi, Takashi Kamakura, Hiroyuki Osada
Abstract
ABSTRACT We identified the biosynthetic gene cluster for lucilactaene, a cell cycle inhibitor from a filamentous fungus Fusarium sp. RK 97–94. The luc1 knockout strain accumulated demethylated analogs, indicating the involvement of Luc1 methyltransferase in lucilactaene biosynthesis. Lucilactaene showed potent antimalarial activity. Our data suggested that methylation and ether ring formation are essential for its potent antimalarial activity.
Topics & Concepts
Gene clusterBiosynthesisFusariumMethyltransferaseFungusAcetylationGeneBiochemistryBiological activityMethylationBiologyStrain (injury)ChemistryStereochemistryIn vitroGeneticsBotanyAnatomyMicrobial Natural Products and BiosynthesisInsect Resistance and GeneticsFungal Biology and Applications