Microfluidic post method for 3-dimensional modeling of platelet–leukocyte interactions
Lining Arnold Ju, Sabine Kossmann, Yunduo Charles Zhao, Laura Moldovan, Yingqi Zhang, Savindi De Zoysa Ramasundara, Fangyuan Zhou, Hang Lu, Imala Alwis, Simone M. Schoenwaelder, Yuping Yuan, Shaun P. Jackson
Abstract
stationary adhesion behavior of single neutrophils and demonstrated the efficacy of the P-selectin and Mac-1 blocking antibodies to reduce neutrophil recruitment and stationary adhesion, respectively. Moreover, the geometry of posts had a major influence on the efficiency of neutrophil recruitment and adhesion stability. This new post method highlights the importance of platelet 3D geometries in facilitating efficient, localized neutrophil recruitment. These findings have potentially important implications for the potent proinflammatory function of microvascular platelet thrombi.