The bacterial cytochrome P450 (CYP) CYP125 enzymes can competitively oxidise sitosterol in the presence of cholesterol
Daniel Z. Doherty, Amna Ghith, Ava Ho, James J. De Voss, Stephen G. Bell
Abstract
. Various other mycobacteria degrade not only cholesterol but plant sterols such as sitosterol and campesterol. In this work we demonstrate that the cytochrome P450 (CYP) CYP125 enzyme family is capable of sitosterol and campesterol side-chain oxidation and activation in these bacteria. We also show that the CYP142 and CYP124 cholesterol hydroxylating enzyme families are significantly less active for sitosterol hydroxylation compared to CYP125 enzymes.
Topics & Concepts
Cytochrome P450ChemistryEnzymeCytochromeBiochemistryCholesterolSteroid Chemistry and BiochemistryPharmacogenetics and Drug MetabolismHormonal Regulation and Hypertension