Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids <i>via</i> a single-cell biocatalytic Δ<sup>1</sup>-dehydrogenation and C17β-carbonyl reduction cascade
Yajiao Zhang, Minjie Liu, Zixin Yang, Juan Lin, Zedu Huang, Fen‐Er Chen
Abstract
Asymmetric synthesis of (+)-boldenone and (+)-boldenone undecylenate was achieved in batch and continuous flow, using an enzyme cascade process catalyzed by an engineered Δ 1 -KstD and a mined 17β-CR.
Topics & Concepts
DehydrogenationChemistryCascadeAnabolismCombinatorial chemistryCatalysisEnantioselective synthesisContinuous flowCascade reactionFlow chemistryBiocatalysisReduction (mathematics)ChromatographyOrganic chemistryBiochemistryReaction mechanismBiochemical engineeringEngineeringMathematicsGeometrySteroid Chemistry and BiochemistryPharmacogenetics and Drug MetabolismPlant biochemistry and biosynthesis