Litcius/Paper detail

PharmaFormer predicts clinical drug responses through transfer learning guided by patient derived organoid

Yuru Zhou, Quanhui Dai, Yanming Xu, Shuang Wu, Minzhang Cheng, Bing Zhao

2025npj Precision Oncology17 citationsDOIOpen Access PDF

Abstract

A major challenge in effective cancer treatment is the variability of drug responses among patients. Patient-derived organoids greatly preserve the genetic and histological characteristics even the drug sensitivities of primary tumor tissues, therefore provide a compelling approach to predict clinical outcome. However, the individual organoid culture and following drug response test are time and cost-consuming, which hinders the potential clinical application. Here, we developed PharmaFormer, a clinical drug response prediction model based on custom Transformer architecture and transfer learning. PharmaFormer was initially pre-trained with the abundant gene expression and drug sensitivity data of 2D cell lines, and was then finalized through a model further fine-tuned with the limited organoid pharmacogenomic data accumulated at the present stage. Our results demonstrate that PharmaFormer, integrating both pan-cancer cell lines and organoids of a specific type of tumor, provides a dramatically improved accurate prediction of clinical drug response. This study highlights that advanced AI models combined with biomimetic organoid models will accelerate precision medicine and future drug development.

Topics & Concepts

OrganoidPharmacogenomicsDrug responseDrugPrecision medicineMedicineComputational biologyDrug developmentComputer scienceBioinformaticsPharmacologyBiologyPathologyNeuroscienceCancer Genomics and DiagnosticsCancer Cells and MetastasisComputational Drug Discovery Methods