Grafting of anti-nucleolin aptamer into preformed and remotely loaded liposomes through aptamer-cholesterol post-insertion
Hamdi Nsairat, Ismail Sami Mahmoud, Fadwa Odeh, Duaa Abuarqoub, Hafsa Al-Azzawi, Rand Zaza, Malak I. Qadri, Said I. Ismail, Abeer Al Bawab, Abdalla Awidi, Walhan Alshaer
Abstract
in both cell lines. Overall, our results demonstrate a successful post-insertion of cholesterol-tagged aptamer into liposomes and the possible combination between active loading and active targeting.
Topics & Concepts
AptamerLiposomeAgarose gel electrophoresisFlow cytometryCytotoxicityConfocal microscopyBiophysicsChemistryNucleolinMolecular biologyCell biologyBiochemistryIn vitroCytoplasmBiologyNucleolusGeneAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryNanoparticle-Based Drug Delivery