Enantioselective UHPLC Screening Combined with <i>In Silico</i> Modeling for Streamlined Development of Ultrafast Enantiopurity Assays
Gioacchino Luca Losacco, Heather Wang, Imad A. Haidar Ahmad, Jimmy DaSilva, Alexey A. Makarov, Ian Mangion, Francesco Gasparrini, Michael Lämmerhofer, Daniel W. Armstrong, Erik L. Regalado
Abstract
second-degree polynomial regression fit using LC simulator (ACD/Labs) software. The retention models were found to be very accurate for chiral resolution of single and multicomponent mixtures of enantiomeric species across different types of CSPs, with differences between experimental and simulated retention times of less than 0.5%. Finally, we illustrate how this approach lays the foundation for a streamlined development of ultrafast enantioseparations applied to high-throughput enantiopurity analysis and its use in the second dimension of two-dimensional liquid chromatography experiments.