Hybrid Pectin-Liposome Formulation against Multi-Resistant Bacterial Strains
Lígia Nunes de Morais Ribeiro, Eneida de Paula, Daise Aparecida Rossi, Guilherme Paz Monteiro, Edson Campos Valadares, Rogério Reis Silva, Rodrigo Rodrigues Franco, Foued Salmen Espíndola, Luíz Ricardo Goulart, Belchiolina Beatriz Fonseca
Abstract
This work describes the development of a gastroresistant antimicrobial formulation composed of two carriers, pectin and liposomes, intended to improve the efficiency of norfloxacin (NOR) against multi-resistant bacterial strains. The formulations showed physicochemical stability for 180 days (4 °C) in terms of size, polydispersity, and zeta potential of the vesicles, prolonging the in vitro release of NOR for 11 h. The hybrid nanocarriers improved the in vitro antimicrobial activity against different multidrug-resistant bacterial strains, such as Salmonella sp., Pseudomonasaeruginosa, E. coli and Campylobacterjejuni, in comparison to commercial NOR and liposomal suspensions. The in vivo toxicity assay in chicken embryos revealed that the hybrid systems were not toxic in any of the different parameters analyzed, a result also corroborated by the analyses of biochemical biomarkers of the chicken-embryos liver function.