Tea polysaccharides from <i>Camellia sinensis</i>: chemical analysis, structural characterization, and inhibition of HeLa cells activity
Yuefang Gao, Yancheng Wang, Xuening Ji, Yao Xiao, Bin Xiao, Pai Peng
Abstract
Tea polysaccharides (TPS) were analyzed and characterized by HPAEC, GPC, FT-IR, AFM, HSQC, and HMBC NMR as well as methylation. The TPS mainly consisted of neutral (F0, F0.2, and F0.3) and acidic polysaccharides (F0.1) rich in glucuronic acid. Moreover, anti-proliferative activity of the TPS on HeLa cells was investigated. It was found that F0 exhibited the highest inhibition rate (36.7%) with 200 µg/ml treatment for 48 h. Structural characterization revealed that F0 had a backbone chain of (1→4)-β-d-Glcp, (1→6)-α-d-Galp, (1→2)-α-l-Rhap and (1→2,4)-α-l-Rhap residues with (1→6)-α-d-Galp and acetyl group side chains.
Topics & Concepts
PolysaccharideHeLaHeteronuclear single quantum coherence spectroscopyCamellia sinensisGlucuronic acidSide chainChemistryBackbone chainChemical structureMethylationCamelliaStereochemistryTwo-dimensional nuclear magnetic resonance spectroscopyBiochemistryOrganic chemistryIn vitroDNABiologyBotanyPolymerPolysaccharides and Plant Cell WallsPolysaccharides Composition and ApplicationsTea Polyphenols and Effects