The Impact of Vitamin E Supplementation on Oxidative Stress, Cognitive Functions, and Aging‐Related Gene Expression in Aged Mice
Farnoosh Molavi Vasei, Mohammad Yasin Zamanian, Maryam Golmohammadi, Mehdi Mahmoodi, Morteza Khademalhosseini, Tayyebeh Tavakoli, Ozra Sadat Esmaeili, Sadegh Zarei, Mohammad Reza Mirzaei, Mohammad Reza Hajizadeh
Abstract
This study investigated the effects of different doses of vitamin E on oxidative stress, cognitive function, and gene expression in aged mice. A total of 32 male mice, aged 12 months, were divided into a control group and three treatment groups. These groups received varying daily doses of vitamin E for a period of 28 days. The results showed significant improvements in cognitive function, specifically in working memory and spatial learning, in the groups that received vitamin E (100, 200, or 400 mg/kg) compared to the control group. The markers of oxidative stress and antioxidant enzyme activities also demonstrated improvements, with higher doses of vitamin E showing greater effects. The analysis of gene expression revealed increased expression of SIRT1, Nrf2, and Calstabin2, particularly at higher doses of vitamin E. These findings suggest that vitamin E supplementation may help counteract age-related cellular changes. The study concludes that vitamin E supplementation can reduce oxidative stress, enhance cognitive function, and affect genetic markers of aging in mice, which may have therapeutic benefits in addressing age-related cognitive decline and oxidative damage. Further research is necessary to investigate the clinical implications of these findings in humans.