An Investigation on the <i>In Vitro</i> Wound Healing Activity and Phytochemical Composition of <i>Hypericum pseudolaeve</i> N. Robson Growing in Turkey
Bahar Kaptaner İğci, Zekí Aytaç
Abstract
OBJECTIVES: N. Robson obtained by two different methods as well as its cytotoxicity, antioxidant activity, and selected phytochemical constituents. MATERIALS AND METHODS: wound healing model. In addition, the chemical content of the species was determined by high pressure liquid chromatography (HPLC). RESULTS: The results of the cytotoxicity assay indicated that the methanolic and aqueous extract did not have any cytotoxic effect on fibroblast cells at concentrations up to 500 μg/mL. Fibroblast migration was significantly increased by 62 μg/mL concentration of the aqueous extracts compared to the negative control. The extracts showed good antioxidant activity and 16 phytochemical compounds were detected by HPLC, with the highest amount for epicatechin. CONCLUSION: extracts have wound healing potential and contain several important antioxidant phenolic compounds. This species deserves further investigation aiming to isolate and identify the active compounds.