Fluorescent labelling as a tool for identifying and quantifying nanoplastics
Patricia Merdy, Adrien Bonneau, Floriane Delpy, Yves Lucas
Abstract
the analysis of 3D fluorescence spectra post-staining. Four fluorescent molecules were tested: cyanine-3 phosphoramidite, rhodamine-6G, fluorescein sodium salt, and Vat Red 15. Our observations indicated that adsorption onto nanoplastic particles resulted in peak shifts in the fluorescence signal, providing sufficient specificity for nanoplastic identification. Among the tested fluorophores, fluorescein was the most effective, successfully discriminating PP, PVC, HDPE, LDPE, and PS. Rhodamine-6G produced shifted signals for HDPE, LDPE, and PS but grouped them together. Cyanine-3 effectively distinguished PVC, PS, and PET, while Vat Red was only able to discriminate PVC.
Topics & Concepts
LabellingFluorescenceMicroplasticsLow-density polyethyleneFluorescent labellingNanotechnologyComputer scienceMaterials scienceChemistryPolyethyleneEnvironmental chemistryComposite materialPhysicsBiochemistryQuantum mechanicsMicroplastics and Plastic PollutionRecycling and Waste Management TechniquesGraphene and Nanomaterials Applications