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Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli

Ryan R. Cupo, James Shorter

2020BIO-PROTOCOL23 citationsDOIOpen Access PDF

Abstract

assays requiring TEV protease.

Topics & Concepts

Tobacco etch virusCLPBBiologyProteaseTandem affinity purificationRecombinant DNABiochemistryMolecular biologyVirusEscherichia coliVirologyEnzymePlant virusGenePotyvirusAffinity chromatographyMitochondrial Function and PathologyRNA modifications and cancerEndoplasmic Reticulum Stress and Disease