A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation
Maria Rodriguez-Rios, Gloria Garoffolo, G Rinaldi, Alicia Megía-Fernández, Silvia Ferrari, Calum T. Robb, Adriano G. Rossi, Maurizio Pesce, Mark Bradley
Abstract
A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.
Topics & Concepts
Neutrophil extracellular trapsInflammationPeptideExtracellularChemistryBiochemistryImmunologyMedicineNeutrophil, Myeloperoxidase and Oxidative MechanismsNitric Oxide and Endothelin EffectsS100 Proteins and Annexins