Electrochemical Detection of a Breast Cancer Biomarker with an Amine-Functionalized Nanocomposite Pt–Fe<sub>3</sub>O<sub>4</sub>–MWCNTs–NH<sub>2</sub> as a Signal-Amplifying Label
Sunil M. Patil, Vijay C. Karade, Jin Hyeok Kim, Ashok D. Chougale, Prashant Patil
Abstract
We report an ultrasensitive sandwich-type electrochemical immunosensor to detect the breast cancer biomarker CA 15-3. Amine-functionalized composite of reduced graphene oxide and Fe 3 O 4 nanoparticles (MRGO-NH 2 ) was used as an electrochemical sensing platform material to modify the electrodes. The nanocomposite comprising Pt and Fe 3 O 4 nanoparticles (NPs) anchored on multiwalled carbon nanotubes (Pt–Fe 3 O 4 –MWCNTs–NH 2 ) was utilized as a pseudoenzymatic signal-amplifying label. Compared to reduced graphene oxide, the composite MRGO-NH 2 platform material demonstrated a higher electrochemical signal. In the Pt–Fe 3 O 4 –MWCNTs–NH 2 label, multiwalled carbon nanotubes provided the substratum to anchor abundant catalytic Pt and Fe 3 O 4 NPs. The nanocomposites were thoroughly characterized using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy. An electroanalytical study and prevalidation of the immunosensor was carried out. The immunosensor exhibited exceptional capabilities in detecting CA 15-3, offering a wider linear range of 0.0005–100 U mL –1 and a lower detection limit of 0.00008 U mL –1 . Moreover, the designed immunosensor showed good specificity, reproducibility, and acceptable stability. The sensor was successfully applied to analyze samples from breast cancer patients, yielding reliable results.