A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
Jacob F. Wardman, Lyann Sim, Jennifer Liu, Teresa A. Howard, Andreas Geissner, Phillip M. Danby, A.B. Boraston, Warren W. Wakarchuk, Stephen G. Withers
Topics & Concepts
GlycoproteinGlycosylationBiochemistryGlycosyltransferaseEnzymeProtein engineeringDirected evolutionFörster resonance energy transferHigh-throughput screeningMucinGlycanEscherichia coliChemistryBiologyFluorescenceGeneMutantPhysicsQuantum mechanicsGlycosylation and Glycoproteins ResearchCarbohydrate Chemistry and SynthesisMonoclonal and Polyclonal Antibodies Research