Enhancing Molecular‐Level Biological Monitoring with a Smart Self‐Assembling <sup>19</sup>F‐Labeled Probe
Zhenchuang Xu, Chenyang Wang, Shengyuan He, Jian Wu, Yanchuan Zhao
Abstract
Abstract Real‐time monitoring of molecular transformations is crucial for advancements in biotechnology. In this study, we introduce a novel self‐assembling 19 F‐labeled nuclear magnetic resonance (NMR) probe that disassembles upon interaction with various nucleotides. This interaction not only activates the 19 F signals but also produces distinct signatures for each specific component, thereby enabling precise identification and quantification of molecules in evolving samples. We demonstrate the capability of this probe for real‐time monitoring of adenosine triphosphate (ATP) hydrolysis and screening potential enzyme inhibitors. These applications highlight the probe's significant potential in enzyme analysis, drug development, and disease diagnostics.