Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures
Stina Oredsson, Atena Malakpour‐Permlid, Johan Zhu, Tilo Weber
Abstract
The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells. • Steps for routine culturing of cells with Oredsson universal replacement (OUR) medium • Guidelines for thawing cells to obtain high viability and recovery in OUR medium • Guidelines for freezing cells to maintain viability • Proof-of-principle studies demonstrated for VERO, A549, THP-1, and Jurkat cells Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.