Monitoring Changes in Biochemical and Biomechanical Properties of Collagenous Tissues Using Label-Free and Nondestructive Optical Imaging Techniques
Tanveer Ahmed Shaik, João L. Lagarto, Enrico Baria, Melis Goktas, Patrick Igoche Onoja, Kerstin G. Blank, Francesco S. Pavone, Jürgen Popp, Christoph Krafft, Riccardo Cicchi
Abstract
. Non-negative least-squares (NNLS) modeling quantifies collagen loss and relative increase of elastin. The Young's modulus, derived from atomic force microscope-based nanoindentation experiments, showed a rapid decrease within the first 8 h of collagen degradation, whereas more gradual changes were observed for optical modalities. We conclude that optical imaging techniques like SHG, RS, and FLIM can monitor collagen degradation in a label-free manner and coarsely access mechanical properties in a nondestructive manner.
Topics & Concepts
ChemistryElastinRaman spectroscopyFluorescenceFluorescence-lifetime imaging microscopyDegradation (telecommunications)NanoindentationSecond-harmonic generationBiophysicsMicroscopyAnalytical Chemistry (journal)Biomedical engineeringNuclear magnetic resonanceLaserOpticsMaterials scienceComposite materialPathologyChromatographyPhysicsTelecommunicationsBiologyComputer scienceMedicineCollagen: Extraction and CharacterizationTendon Structure and TreatmentLaser Applications in Dentistry and Medicine