A method to determine reactive oxygen species production in intestinal and liver cell cultures using the 2′,7′-dichlorodihydrofluorescein diacetate assay
Ilzé Engelbrecht, Suranie Horn, John P. Giesy, Rialet Pieters
Abstract
in intestinal and liver cells.•This method is inexpensive and can be easily performed in standard laboratories.•The method provides a tool for the high-throughput screening of environmental samples.
Topics & Concepts
Reactive oxygen speciesBioassayOxidative stressXenobioticFlow cytometryChemistryIntracellularIn vitroBiochemistryBiologyMolecular biologyEnzymeGeneticsCarcinogens and Genotoxicity AssessmentToxic Organic Pollutants ImpactAir Quality and Health Impacts