Microfluidic synthesis of iron oxide nanoparticles for highly efficient intracellular delivery in stem cells and cancer cells
Athira Prasad, R. Gayathri, B. Nandhini, R. Jayaganthan, Srabani Kar, Tuhin Subhra Santra
Abstract
, which helps to create transient cell membrane pores and deliver small to large biomolecules into cells by a simple diffusion process. We carried out highly efficient intracellular delivery using propidium iodide (PI) (668 Da), dextran (3 kDa), 6159 bp pcDNA3-enhanced green fluorescent protein (EGFP) and Cy-5-β-galactosidase enzyme (465 kDa) into mouse fibroblast (L929), human cervical (SiHa) cancer cells, LN229, a human glioblastoma cell line, and human mesenchymal stem cells (hMSCs). The best results achieved for Cy-5-β-galactosidase enzyme transfection in hMSCs were 98.6% transfection efficiency and 98.6% cell viability. Thus, our platform might have potential applications in cell therapeutics and diagnostics.