Stress tolerance in entomopathogenic nematodes: Engineering superior nematodes for precision agriculture
Dorothy Maushe, Vera Ogi, Keerthi Divakaran, Arletys María Verdecia Mogena, Paul Himmighofen, Ricardo A. R. Machado, Benjamin D. Towbin, Ralf‐Udo Ehlers, Carlos Molina, Christian Parisod, Christelle A. M. Robert
Abstract
Entomopathogenic nematodes (EPNs) are soil-dwelling parasitic roundworms commonly used as biocontrol agents of insect pests in agriculture. EPN dauer juveniles locate and infect a host in which they will grow and multiply until resource depletion. During their free-living stage, EPNs face a series of internal and environmental stresses. Their ability to overcome these challenges is crucial to determine their infection success and survival. In this review, we provide a comprehensive overview of EPN response to stresses associated with starvation, low/elevated temperatures, desiccation, osmotic stress, hypoxia, and ultra-violet light. We further report EPN defense strategies to cope with biotic stressors such as viruses, bacteria, fungi, and predatory insects. By comparing the genetic and biochemical basis of these strategies to the nematode model Caenorhabditis elegans, we provide new avenues and targets to select and engineer precision nematodes adapted to specific field conditions.