Analysis of the Contribution of <i>cyp51</i> Genes to Azole Resistance in <i>Aspergillus</i> Section <i>Nigri</i> with the CRISPR-Cas9 Technique
Alba Pérez‐Cantero, Adela Martín‐Vicente, Josep Guarro, Jarrod R. Fortwendel, Javier Capilla
Abstract
Cyp51 contribution to azole resistance has been broadly studied and characterized in Aspergillus fumigatus , whereas it remains poorly investigated in other clinically relevant species of the genus, such as those of section Nigri . In this work, we aimed to analyze the impact of cyp51 genes ( cyp51A and cyp51B ) on the voriconazole (VRC) response and resistance of Aspergillus niger and Aspergillus tubingensis .
Topics & Concepts
CRISPRAzoleBiologyAspergillusGeneGeneticsSection (typography)Computational biologyMicrobiologyAntifungalComputer scienceOperating systemAntifungal resistance and susceptibilityFungal Infections and StudiesStudies on Chitinases and Chitosanases