Self-decorating cells <i>via</i> surface-initiated enzymatic controlled radical polymerization
Andrea Belluati, Dominic Happel, Malte Erbe, Nicole Kirchner, Anna Szelwicka, A. Bloćh, Valeria Berner, Andreas Christmann, Brigitte Hertel, Raheleh Pardehkhorram, Amin Reyhani, Harald Kolmar, Nico Bruns
Abstract
cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.
Topics & Concepts
Bioorthogonal chemistryHorseradish peroxidasePolymerizationPolymerYeastSaccharomyces cerevisiaeSurface modificationChemistryPeroxidaseCombinatorial chemistryEnzymePolymer chemistryNanotechnologyMaterials scienceBiochemistryOrganic chemistryClick chemistryPhysical chemistryPolymer Surface Interaction StudiesClick Chemistry and Applicationsbiodegradable polymer synthesis and properties