A facile aptamer immobilization strategy to fabricate a robust affinity monolith for highly specific in-tube solid-phase microextraction
Tingting Zhao, Shiqian Tong, Susu Zhou, Chenchen Lin, Xucong Lin, Zenghong Xie
Abstract
An ultra-high density aptamer immobilization of up to 8600 pmol μL −1 was achieved via a simple adsorption strategy, and excellent specific recognition and anti-cross reactivity in IT-SPME were realized.
Topics & Concepts
MonolithAptamerSolid-phase microextractionChromatographyTube (container)ChemistryPhase (matter)Materials scienceNanotechnologyCatalysisGas chromatography–mass spectrometryMass spectrometryComposite materialOrganic chemistryBiologyGeneticsAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionAnalytical chemistry methods development