A triphenylamine-based fluorescence probe for detection of hypochlorite in mitochondria
Guan-Hua Yu, Haoran Hu, Ruibin Liu, Guo-Zhu Sheng, Jia-Jie Niu, Ying Fang, Kun‐Peng Wang, Zhi‐Qiang Hu
Abstract
The level of HClO/ClO − in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO − in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO − . The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO − . Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO − and its detection limit was calculated as 10.5 μM. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/exogenous ClO − levels in the mitochondria of living cells.