Proteome Dynamics during Antibiotic Persistence and Resuscitation
Maja Šemanjski, Fabio Lino Gratani, Till Englert, Payal Nashier, Viktor Beke, Nicolas Nalpas, Elsa Germain, Shilpa Elizabeth George, Christiane Wolz, Kenn Gerdes, Boris Maček
Abstract
to devise a generic method for temporal analysis of protein synthesis during toxin-induced persistence and antitoxin-mediated resuscitation. Our time-resolved, 24-h measurement of label incorporation demonstrated low but widespread protein synthesis during persistence. At the onset of resuscitation, cells restored translation machinery and resumed metabolism by inducing glycolysis and biosynthesis of amino acids. Our study provides the first global analysis of protein synthesis in persisting and resuscitating bacterial cells, and as such, presents an unprecedented resource to study the processes governing antibiotic persistence.