Alginate-pectin microparticles embedding self-assembling antimicrobial peptides and resveratrol for antimicrobial and anti-inflammatory applications
Cesar Augusto Roque‐Borda, Marco Roberto Chávez-Morán, Laura Maria Duran Gleriani Primo, José Carlos Estanislao Márquez Montesinos, Vinicius Martinho Borges Cardoso, Mauro de Mesquita Souza Saraiva, Caroline Maria Marcos, Marlus Chorilli, Fernando Alberício, Beatriz G. de la Torre, Fernando R. Pavan, Andréia B. Menguin
Abstract
Integrating antimicrobial peptides (AMPs) into alginate-pectin-based microstructured delivery systems enhances the controlled release and stability of bioactive like resveratrol. This study examines spray-dried microparticles utilizing alginate and pectin as primary carbohydrate polymers combined with aggregated AMPs for dual antioxidant and antimicrobial effects. The AMP was self-assembled into nanostructures upon interaction with alginate and pectin, which were subsequently incorporated into spray-dried microparticles, resulting in a stable delivery system. In silico modeling and in vitro release studies under simulated gastrointestinal conditions showed that AMP-containing microparticles provided a slower, sustained resveratrol release than peptide-free systems. The Weibull model best described the release, indicating a multi-phase behavior driven by diffusion and erosion. In gastric simulated conditions, the alginate-pectin matrices with AMP improved structural integrity, reducing release, while in intestinal ones, partial erosion enabled controlled release. In vivo infection studies using Galleria mellonella demonstrated significant reductions in inflammation and bacterial load 48 h post-infection. These results suggest that alginate-pectin microparticles with aggregated AMPs enhance the antioxidant release and maintain antimicrobial activity, making them promising for gastrointestinal applications. According to these promising findings, the next step studies will aim to enhance site-specific therapeutic performance through formulation refinement and targeted delivery strategies. • Antimicrobial peptides self-assemble upon interaction with alginate and pectin. • Spray drying produced microparticles with improved structural and functional stability. • Microencapsulation with peptide/resveratrol enhances antimicrobial and anti-inflammatory potential. • The delivery system provides pH-responsive and sustained release in simulated gastrointestinal fluids. • In vivo assays in Galleria mellonella confirmed reduced bacterial load and inflammation.