An RNA in situ hybridization protocol optimized for monocot tissue
Nora R. Zöllner, Margaret Bezrutczyk, Reinout Laureyns, Hilde Nelissen, Rüdiger Simon, Wolf B. Frommer
Abstract
hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections. For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021).
Topics & Concepts
In situ hybridizationIn situComputational biologyRNABiologyMolecular biologyGeneticsChemistryMessenger RNAGeneOrganic chemistryPlant Molecular Biology ResearchPlant Reproductive BiologyPlant nutrient uptake and metabolism