Investigating the cellular antioxidant and anti-inflammatory effects of the novel peptides in lingzhi mushrooms
Thanyaporn Aursuwanna, Sajee Noitang, Papassara Sangtanoo, Piroonporn Srimongkol, Tanatorn Saisavoey, Songchan Puthong, Onrapak Reamtong, Aphichart Karnchanatat
Abstract
sub-fraction. DRVSIYGWG and ALLSISSF were discovered as new peptides with different antioxidant properties. Adenocarcinoma colon (Caco-2) cells showed the antioxidant action of these synthesized peptides. This activity was linked to peptide concentration. The peptides and their pure synthetic counterparts were found to reduce NO generation by RAW 264.7 macrophages without causing cytotoxicity. The results of gene expression reveal that the DRVSIYGWG and ALLSISSF peptides were able to cut the expression of the proinflammatory cytokine genes iNOS, IL-6, TNF-α, and COX-2 in the context of RAW 264.7 macrophages.