Deep eutectic solvents as a stabilising medium for NAD coenzyme: unravelling the mechanism behind coenzyme stabilisation effect
Mia Radović, Lucija Hok, Manuela Panić, Marina Cvjetko Bubalo, Robert Vianello, Marijana Vinković, Ivana Radojčić Redovniković
Abstract
DES choline chloride:urea effectively stabilises NAD coenzyme up to 50 days at room temperature. Consequently, this solvent could be used to minimise NAD degradation during storage, assays, and enzyme-catalysed oxidoreductive reactions.
Topics & Concepts
NAD+ kinaseCofactorChemistryDeep eutectic solventCholine chlorideCoenzyme AEutectic systemUreaSolventEnzymeBiochemistryOrganic chemistryInorganic chemistryReductaseAlloyTryptophan and brain disordersIonic liquids properties and applicationsElectrochemical sensors and biosensors