Self-Renewable Tag for Photostable Fluorescence Imaging of Proteins
Zhichao Du, Wenjing Wang, Siyuan Luo, Lingjie Zhang, Shuowei Yuan, Yongzhen Hei, Zhangbin Bao, Chunlai Chen, Yi Lin, Ling Chu
Abstract
We report the development of a self-renewable tag (srTAG) for protein fluorescence imaging. srTAG leverages the "on-protein" fluorophore equilibrium between the fluorescent zwitterion and non-fluorescent spirocyclic form and the reversible fluorescence labeling to enable self-recovery of fluorescence after photobleaching. This small-sized srTAG allows 2-6 times longer imaging duration compared to other commonly used self-labeling tags and is compatible with fluorophores with different spectral properties. This study provides a new strategy for fine tuning of self-labeling tags.
Topics & Concepts
ChemistryFluorophoreFluorescencePhotobleachingFluorescence recovery after photobleachingFluorescence-lifetime imaging microscopyZwitterionFluorescence in the life sciencesFluorescent labellingBiophysicsMoleculeBiochemistryOpticsOrganic chemistryPhysicsMembraneBiologyClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniquesAdvanced Biosensing Techniques and Applications