Amplification of Femtograms of Bacterial DNA Within 3 h Using a Digital Microfluidics Platform for MinION Sequencing
Yuguang Liu, Patricio Jeraldo, Helena Mendes‐Soares, Thao Masters, Abigail E. Asangba, Heidi Nelson, Robin Patel, Nicholas Chia, Marina Walther-António
Abstract
DNA in a mock community DNA sample and characterize the limit of bacterial detection in the presence of human cells. This approach can be used to identify microbes with minute amounts of genetic material in samples depleted of human cells within 3 h.
Topics & Concepts
MinionNanopore sequencingCorynebacterium glutamicumMultiple displacement amplificationMicrofluidicsDNADNA sequencingDigital polymerase chain reactionBiologyComputational biologyDNA extractionGenomePolymerase chain reactionGeneticsBacteriaGeneNanotechnologyMaterials scienceElectrowetting and Microfluidic TechnologiesMicrofluidic and Capillary Electrophoresis ApplicationsBiosensors and Analytical Detection