Novel Approach to Overcome Defects of Cell-SELEX in Developing Aptamers against Aspartate β-Hydroxylase
Hadi Bakhtiari, Abbas Ali Palizban, Hossein Khanahmad, Mohammad Reza Mofid
Abstract
values of 47.51, 39.38, and 65.23 nM, respectively, were obtained, while AP-Cell 1 and 3 could then successfully spot ASPH displayed on the tissues. Our study showed that the Counter-SELEX could be considered as a complementary method for Cell-SELEX to overcome the imperfectness of the negative selection step. Moreover, high-throughput nucleotide sequencing could help to shorten the overall process.
Topics & Concepts
Systematic evolution of ligands by exponential enrichmentAptamerComputational biologyOligonucleotideFlow cytometrySELEX Aptamer TechniqueBiologySelection (genetic algorithm)ChemistryMolecular biologyBiochemistryDNAComputer scienceGeneRNAArtificial intelligenceAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryBiosensors and Analytical Detection