Chemical Composition and Antimicrobial Activity of Essential Oils from the Leaves and Stems of <i>Tinomiscium petiolare</i> Hook.f. & Thomson from Vietnam
B. B. Thinh, Roman V. Doudkin, Le D. Chac, Hoang Van Chinh, Nguyễn Thị Hồng, William N. Setzer, Isiaka A. Ogunwande
Abstract
Essential oils from the leaves and stems of Tinomiscium petiolare Hook.f. & Thomson were obtained by hydrodistillation and the chemical components were determined by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS). Also, the microdilution broth assay was used to test the antimicrobial activity of essential oils. The respective yields of the T. petiolare leaf and stem essential oils were 0.06 % and 0.04 % (v/w), calculated on a dry weight basis. The major components (percentages, respectively) identified from the leaf and stem essential oils of T. petiolare were relatively similar, comprising of β-bisabolene (11.43 % and 13.80 %), trans-α-bergamotene (6.52 % and 6.61 %), geranyl acetate (6.12 % and 5.77 %), (E)-caryophyllene (5.04 % and 4.73 %), δ-cadinene (4.10 % and 5.27 %), (E,E)-α-farnesene (4.54 % and 4.65 %) and α-zingiberene (3.85 % and 4.50 %). The antimicrobial activity test indicated that T. petiolare stem essential oil displayed moderate activity against Staphylococcus aureus ATCC 6538 with Minimum inhibitory concentration (MIC) value of 200 μg/mL, while both essential oils inhibited the growth of Pseudomonas aeruginosa ATCC 9027 with MIC value of 200 μg/mL. The chemical components and antimicrobial activity of the T. petiolare leaf and stem oils are reported for the first time.