Human iPSC-engineered cardiac tissue platform faithfully models important cardiac physiology
Willem J. de Lange, Emily T. Farrell, Caroline R. Kreitzer, Derek R. Jacobs, Di Lang, Alexey V. Glukhov, J. Carter Ralphe
Abstract
This study seeks to provide an in-depth assessment of contractile performance of human iPSC-derived cardiomyocytes cultured together with fibroblasts in a 3-dimensional-engineered tissue and compares performance both over time as cells mature, and with corresponding measures found in the literature using alternative 3D culture configurations. The suitability of 3D-engineered human cardiac tissues to model cardiac function is emphasized, and data provided to assist in the selection of the most appropriate configuration based on the target application.
Topics & Concepts
Induced pluripotent stem cellHuman Induced Pluripotent Stem CellsEmbryonic stem cellCell biologyCalciumBiologyCalcium in biologyNeuroscienceComputational biologyInternal medicineIntracellularMedicineGeneBiochemistryTissue Engineering and Regenerative MedicinePluripotent Stem Cells Research3D Printing in Biomedical Research