Rasusa: Randomly subsample sequencing reads to a specified coverage

Abstract

A fundamental requirement for many applications in genomics is the sequencing of genetic material (DNA/RNA). Different sequencing technologies exist, but all aim to accurately reproduce the sequence of nucleotides (the individual units of DNA and RNA) in the genetic material under investigation. The result of such efforts is a text file containing the individual fragments of genetic material -termed "reads" -represented as strings of letters (A, C, G, and T/U).

Topics & Concepts