NAT10-mediated ac4C modification promotes stemness and chemoresistance of colon cancer by stabilizing NANOGP8
Liping Gao, Ting-Dong Li, Su-Zhen Yang, Hui-Min Ma, Xiang Wang, Dekui Zhang
Abstract
Background: C modification mediated by NAT10 in regulating stemness and chemosensitivity in CC remains undetermined. Methods: The levels of NAT10 in normal colon and chemoresistant CC tissues were determined utilizing quantitative real-time polymerase chain reaction alongside immunohistochemistry. Assessing cancer cell stemness and chemosensitivity was conducted by various methods including spheroid and colony formation, western blotting, and flow cytometry. RNA-Seq was used to identify target genes, and RNA immunoprecipitation analysis was used to explore the potential mechanisms. Results: mRNA stability. Conclusions: . The inhibition of NAT10 via Remodelin improves chemotherapeutic efficacy and impedes CC progression.