A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA
Shaoheng Bao, Hui Jiang, Lingyun Zhu, Ge Yao, Penggang Han, Xiukun Wan, Kang Wang, Tianyu Song, Changjun Liu, Shan Wang, Zhe-Yang Zhang, Dongyi Zhang, Er Meng
Abstract
is identified as the most suitable tuning factor for shake flask culture. Moreover, it is shown that dynamic overexpression of the Hfq chaperone is the key to combinatorial repression without disruptions on cell growth. To show a broad applicability, the production titers of pinene, pentalenene, and psilocybin are improved by 365.3%, 79.5%, and 302.9%, respectively, by applying combinatorial dynamic repression.
Topics & Concepts
Psychological repressionQuorum sensingBiologySmall RNACell biologyRNARegulation of gene expressionTranscription factorGeneBiochemistryGene expressionVirulenceTransgenic Plants and ApplicationsMicrobial Metabolic Engineering and BioproductionBacterial Genetics and Biotechnology