A protocol for single nucleus RNA-seq from frozen skeletal muscle
Tyler Soule, Carly Sabine Pontifex, Nicole L. Rosin, Matthew M Joel, Suk‐young Lee, Minh Dang Nguyen, Sameer Chhibber, Gerald Pfeffer
Abstract
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by single-nucleus RNA sequencing. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease.