A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity
David Beriashvili, Ru Yao, Francesca D’Amico, Michaela Krafčíková, Andrei Gurinov, Adil Safeer, Xinyi Cai, Monique P. C. Mulder, Yangping Liu, Gert E. Folkers, Marc Baldus
Abstract
N] labeled ubiquitin. We report that SNAPol-1 passively diffuses and homogenously distributes within whole cells and cell nuclei providing ubiquitin spectra of high sensitivity and remarkably improved spectral resolution. For cell nuclei, physical enrichment facilitates a further 4-fold decrease in measurement time and provides an exclusive structural view of the nuclear ubiquitin pool. Taken together, these advancements enable atomic interrogation of protein conformational plasticity at atomic resolution and with sub-cellular specificity.
Topics & Concepts
Solid-state nuclear magnetic resonanceSolid-stateChemistryField (mathematics)Computational biologyComputer scienceCombinatorial chemistryBiologyNuclear magnetic resonancePhysicsMathematicsPhysical chemistryPure mathematicsAdvanced NMR Techniques and ApplicationsMuon and positron interactions and applicationsBoron and Carbon Nanomaterials Research