Microbiome changes in the gastric mucosa and gastric juice in different histological stages of <i>Helicobacter pylori</i>-negative gastric cancers
Qinghua Sun, Jing Zhang, Yanyan Shi, Jing Zhang, Weiwei Fu, Shigang Ding
Abstract
BACKGROUND: -negative GC (HPNGC). AIM: To characterize microbial profiles of gastric mucosa and juice for HPNGC carcinogenesis and identify distinct taxa in precancerous lesions. METHODS: -negative cases, including 56 superficial gastritis (SG), 9 atrophic gastritis (AG), 27 intestinal metaplasia (IM), 29 dysplasia (Dys), and 13 GC cases, to investigate differences in gastric microbial diversity and composition across the disease stages. In addition, paired gastric mucosa and juice samples from 18 SG, 18 IM, and 18 Dys samples were analyzed. α-Diversity was measured by Shannon and Chao1 indexes, and β-diversity was calculated using partial least squares discrimination analysis (PLS-DA). Differences in the microbial composition across disease stages in different sample types were assessed using the linear discriminant analysis effect size. RESULTS: being more abundant in gastric juice. CONCLUSION: Our results provide insights into potential taxonomic biomarkers for HPNGC and its precancerous stages and assist in predicting the prognosis of IM and Dys based on the mucosal microbiota profile.