Successful batch and continuous lyophilization of mRNA LNP formulations depend on cryoprotectants and ionizable lipids
Alexander Lamoot, Joris Lammens, Emily De Lombaerde, Zifu Zhong, Mark Gontsarik, Yong Chen, T.R.M. De Beer, Bruno G. De Geest
Abstract
mRNA translation into protein, post lyophilization strongly depended on the ionizable lipid in the LNP formulation.
Topics & Concepts
CryoprotectantChemistryChromatographyMessenger RNABiochemistryFood scienceBiologyCryopreservationGeneCell biologyEmbryoProtein purification and stabilityRNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in Insects