Preclinical concept studies showing advantage of an inhaled anti-CTGF/CCN2 protein for pulmonary fibrosis treatment
Vanessa Neiens, Eva-Maria Hansbauer, Thomas J. Jaquin, J. Peper-Gabriel, Poornima Mahavadi, Mark E. Snyder, Maximilian J. Grill, Cornelia Wurzenberger, Antonio Konitsiotis, Adriana Estrada‐Bernal, Kristina Heinig, Athanasios Fysikopoulos, Nicolas Schwenck, Stefan Grüner, Denis Bartoschek, Theresia Mosebach, Sandra Kerstan, Joe A. Wrennall, Marleen Richter, Kentaro Noda, Konrad Höetzenecker, Janette K. Burgess, Robert Tarran, Claudia Wurzenberger, Karl-Robert Wichmann, Jonas Biehler, Kei W. Müller, Andreas Günther, Oliver Eickelberg, Mary Fitzgerald, Shane A. Olwill, Gabriele Matschiner, Marina Pavlidou
Abstract
Inhaled therapeutics have high potential for the treatment of chronic respiratory diseases of high unmet medical need, such as idiopathic pulmonary fibrosis (IPF). Preclinical and early clinical evidence show that cellular communication network factor 2 (CCN2), previously called connective tissue growth factor (CTGF), is a promising target for the treatment of IPF. In recent phase 3 clinical trials, however, systemic CCN2 inhibition failed to demonstrate a clinically meaningful benefit. Here, we present the preclinical profile of the inhaled anti-CCN2 Anticalin® protein PRS-220. Our study demonstrates that efficient pulmonary delivery directly translates into superior efficacy in relevant models of pulmonary fibrosis when compared to systemic CCN2 inhibition. Moreover, we present a holistic approach for the preclinical characterization of inhaled PRS-220 from state-of-the art in vitro and in vivo models to novel human ex vivo and in silico models, highlighting the advantage of inhaled drug delivery for treatment of respiratory disease. This work compares the preclinical lung biodistribution and efficacy profile of inhaled anti-CCN2 (cellular communication network factor 2) Anticalin® protein PRS-220 for the treatment for idiopathic pulmonary fibrosis (IPF) compared to systemic delivery of a CCN2 inhibitor.