An ICT-switched fluorescent probe for visualizing lipid and HClO in lipid droplets during ferroptosis
Leilei Yao, Hongjun Song, Caixia Yin, Fangjun Huo
Abstract
Herein, we have designed and synthesized a fluorescent probe, LDs-ClO, which can detect hypochlorous acid and lipid accumulation simultaneously in lipid droplets of live RAW 264.7 cells. Cell ferroptosis was discovered to lead to an increase in HClO levels, and to possibly further stimulate accumulation of lipid. We expect the results of this work with LDs-ClO to promote the study of physiological and pathological processes related to lipid droplets and hypochlorite.
Topics & Concepts
FluorescenceHypochlorous acidLipid dropletChemistryLipid oxidationBiophysicsNanotechnologyBiochemistryMaterials scienceOpticsBiologyPhysicsAntioxidantLipid metabolism and biosynthesisCholesterol and Lipid MetabolismMass Spectrometry Techniques and Applications