Exploiting the Type I-B CRISPR Genome Editing System in Thermoanaerobacterium aotearoense SCUT27 and Engineering the Strain for Enhanced Ethanol Production
Kaiqun Dai, Hongxin Fu, Xiaolong Guo, Chunyun Qu, Yang Lan, Jufang Wang
Abstract
, using the native CRISPR/Cas system. The engineered strain showed satisfactory performance with improved ethanol productivity from various lignocellulosic hydrolysates. Our data lay the foundations for development of this thermophilic microbe into an excellent ethanol producer using lignocellulosic hydrolysates. The methods described here may also provide a reference to develop multigene editing methods for other microorganisms.
Topics & Concepts
CRISPRGenome editingMetabolic engineeringBiologyChemistryBiochemistryGeneticsGeneCRISPR and Genetic EngineeringMicrobial Fuel Cells and BioremediationInsect symbiosis and bacterial influences