Novel Japanese encephalitis virus NS1-based vaccine: Truncated NS1 fused with E. coli heat labile enterotoxin B subunit
Jiawu Wan, Ting Wang, Jing Xu, Tao Ouyang, Qianruo Wang, Yanni Zhang, Shiqi Weng, Yihan Li, Yu Wang, Xiu Xin, Xiaoling Wang, Sha Li, Lingbao Kong
Abstract
BACKGROUND: Current vaccines against Japanese encephalitis virus (JEV) of flaviviruses have some disadvantages, such as the risk of virulent reversion. Non-structural protein NS1 is conserved among flaviviruses and confers immune protection without the risk of antibody-dependent enhancement (ADE). Therefore, NS1 has become a promising vaccine candidate against flaviviruses. METHODS: and its-induced antisera was evaluated in the mice challenged with JEV by analyzing mortality and morbidity. FINDINGS: -induced antisera provides a protection against JEV infection in mice. INTERPRETATION: could serve as a novel protein-based mucosa vaccine targeting JEV and other flaviviruses. FUNDING: This work was supported by the National Natural Science Foundation, Jiangxi Province Science and Technology Committee, Education Department of Jiangxi Province.